Distribution, purification, and characterization of thermostable phenylalanine dehydrogenase from thermophilic actinomycetes
نویسندگان
چکیده
منابع مشابه
Distribution, purification, and characterization of thermostable phenylalanine dehydrogenase from thermophilic actinomycetes.
Phenylalanine dehydrogenase (L-phenylalanine:NAD oxidoreductase, deaminating; EC 1.4.1.-) was found in various thermophilic actinomycetes. We purified the enzyme to homogeneity from Thermoactinomyces intermedius IFO 14230 by heat treatment and by Red Sepharose 4B, DEAE-Toyopearl, Sepharose CL-4B, and Sephadex G-100 chromatographies with a 13% yield. The relative molecular weight of the native e...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1991
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.173.13.3943-3948.1991